While in the crystal framework of the D273N mutant of NagA, the aspect chain of His-143 is 3.0 Å faraway from the phosphonate oxygen in the mimic on the tetrahedral intermediate (21). This final result indicates this group could facilitate the activation of the carbonyl team in the substrate at the side of an interaction Using the steel ion bound within the active web site.
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there are tyrosine (Y223) and lysine (K139) residues that might also be contributing for the conversation With all the phosphate moiety with the substrate. Deprotonation of possibly of such residues at substantial pH could result in a fall in the worth of k
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At the moment 1.five mL of acetic anhydride was additional and allowed to respond at place temperature overnight. Immediately after elimination with the solvent, the residue was dissolved in 100 mL of chloroform and washed with dilute HCl, saturated NaHCO3 then dried around Na2SO4. The products, diphenyl N
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This observation indicates that There have been a divergence in the evolution of NagA and indicates that there are fundamental dissimilarities while in the mechanistic aspects for substrate activation and hydrolysis. NagA from E. coli
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